ABSTRACT
Aims@#Non-thermal atmospheric-pressure plasma (cold plasma), is described as a partly ionized gas. Cold plasma is a new method of medicine for killing the bacteria, treatment of cancer diseases, accelerates the healing of infectious ulcers, especially in infection caused by Meticillin-resistant Staphylococcus aureus (MRSA). This study aimed to investigate the impact of Non-thermal atmospheric-pressure plasma on MRSA S. aureus organism isolated from burn wound infection in vitro and in vivo. @*Methodology and results@#Five MRSA S. aureus strains were recovered in burn patients from Shahid Motahari Burns Hospital, Tehran, Iran. They confirmed by microbiology and biochemical tests. Antibiotic susceptibility testing was performed using Kirby Bauer Disk Diffusion Method with selected antibiotics. Then, the antibacterial impact of atmospheric non-thermal plasma on MRSA in vitro and in vivo at different times was assessed. After that, the tissue was randomly separated from control and treated mice with plasma and transferred to the Histopathology Laboratory for further evaluation. Results of the inactivation of MRSA by non-thermal atmospheric plasma showed no bacterial growth. Also, results of the impact of non-thermal helium plasma in vivo environment revealed that, in addition to healing in the animal wound, the burn wounds infection was healed and treated according to the histological results. @*Conclusion, significance and impact of study@#Our results confirmed the inactivation of MRSA S. aureus, healing of animal burn wound and complete treatment by non-thermal atmospheric plasma. It recommended that cold plasma can be used for the treatment of burn wounds infection due to the gentle on the human skin.
ABSTRACT
Background: Catheter-associated [CA] bacteriuria is a result of the extensive usage of urinary catheterization. Once a catheter is placed, many patients achieve bacteriuria, even with the use of greatest consideration and care of the catheter. In this study, we decided to evaluate the prevalence of Catheter-associated bacteriuria in patients who received short-term catheterization in the northeast of Iran
Materials and Methods: In this cross-sectional study during one year [among 2014-2015] 275 patients who have admitted recently and have no history of catheterization and drug consumption were included. Three samples were taken from patients before, one day after catheterization and after removal of the catheter. The urine samples were analyzed and cultured on the suitable media. Antibiotics susceptibility testing was performed by disk diffusion method. Then, data analyzed using SPSS software by Student t-test. In addition, the p values less than 0.05 were considered as significant
Results: In general, the rate of catheter-associated bacteriuria in these hospitals was 68% [187 cases of 275]. The mean age of the participants and patients with bacteriuria were 41+/-1.2 and 24.8+/-6.2 years old, respectively. The most common isolated bacteria were Escherichia coli [50.6%] followed by Staphylococcus aureus and Klebsiella pneumonia [21.6%]. The highest sensitivity was reported against kanamycin [68.9%] and highest resistance was observed against ampicillin with a rate of 96.3%
Conclusion: For prevention of healthcare-associated UTI, correct catheterization and use of the closed catheter system is recommended. In addition, before prescribing any antibiotics it should be paying attention to the antibiotics susceptibility testing results
ABSTRACT
The spa gene occurs in all strains of Staphylococcus aureus [S. aureus], can function as a genetic marker and might be used distinguish strains at the species level. Hence, due to these advantages, we used spa typing and the Based Upon Repeat Pattern [BURP] to assign the clonal and phylogenetic relationships of S. aureusstrains. The sensitivity of S. aureus strains to methicillin was determined using agar disk diffusion. The extracted DNA from 56 isolates of S. aureus was subjected to PCR to detect the spa gene with specific primers. The spa typing method was performed for each of the isolates, and then, BURP was used to cluster spa types [spa-CCs]. Finally, using relevant software, the phylogenic tree was drawn. The results of this study showed that 25 out of 56 [44.6%] isolates were resistant to methicillin. The typing of S. aureus isolates revealed 24 different spa types among 56 isolates, and BURP analysis clustered the 24 spa types into 5 spa clonal complexes [CCs] and 12 singletons. The process of spa typing, in combination with BURP analysis, provides an efficient method for investigating phylogenetic and clonal relationships among clinical isolates and can be useful for monitoring bacterial spread between hospitals and communities as well as between and within hospitals